In vitro evaluation of the single and multispecies biofilm prevention capabilities of two wound irrigation solutions and a topical antiseptic
To investigate the biofilm prevention capabilities of 2 wound irrigation solutions and one topical antiseptic using single and mixed species in vitro assays. Material and methods A modified Calgary Biofilm Device (CBD) was used to determine the ability of 3 topical solutions (ActiMaris® (Quantum Medis), Prontosan® wound irrigation solution (B.Braun), and Octenisept ® (Schulke)) to prevent the development of Staphylococcus aureus and Pseudomonas aeruginosa single species and mixed species biofilms. Evidence of biofilm development was assessed at 30 minutes, 3 hours, 24 hours, 48 hours and 72 hours. Biofilm material was recovered by sonication and quantified using colony counts. Results No S. aureus attachment was reported at 30 minutes or 3 hour when samples were treated with the test agents. The topical antiseptic Octenisept ® prevented S. aureus biofilm growth for 72 hours, prevented P. aeruginosa biofilm growth up to 3 hours and continued to demonstrate multispecies biofilm inhibition at 72 hours. ActiMaris®, Prontosan® wound irrigation solution, did not prevent attachment of P. aeruginosa or mixed species biofilms. Conclusion If we accept the definition of a mature biofilm as one that has been forming for more than 10 hours, Octenisept® was the only solution that effectively prevented mature S. aureus biofilm development and mixed species biofilm development.